Research Insights
Establishing an In Vitro Organoid Model to Study Feline Chronic Kidney Disease Pathophysiology
Through this research program, we propose to screen relevant drugs (Dexa/EXA) in canine intestinal organoids {Milestone 1} establish, for the first time, an in vitro model of chronic kidney disease in dogs {Milestone 2} and cats {Milestone 3}. These novel models will then be cultured either with or without TNF-α and ideal study parameters will be established {Milestone 4}. Finally, canine and feline peripheral blood mononuclear cells (PBMCs) will be added to the models to study the effects of dapagliflozin (SGLT-2 inhibitor, DAPA), exenatide (GLP1R agonist, EXA) and spironolactone (mineralocorticoid receptor antagonist, SPIRO) on markers of epithelial integrity and (pro-) inflammatory cytokine expression {Milestone 5}.
Funder: Ceva Sante Animale
Amount: $969,578
PI: Jonathan Mochel, College of Veterinary Medicine